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The loss of action is not really as a consequence of a diminished capacity to bind divalent cations Considering that the steel information of the purified mutants was identical to the wild style enzyme. The catalytic properties of such mutants are totally according to the proposed part of this residue inside the chemical system as the main common foundation for activation of the hydrolytic h2o molecule and the next protonation in the leaving team amine.
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-acetyl-l-aspartate is a very good substrate for aspartoacylase from human brain (31). The kinetic parameters for that hydrolysis of six with NagA are stated in Table 2 This substrate is hydrolyzed in a drastically faster rate than could be the corresponding N
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Web-site precise mutants of NagA had been constructed so that you can ascertain the roles of precise residues in substrate recognition and catalytic purpose. The metallic material along with the kinetic parameters for the mutants of NagA constructed for this investigation are presented in Table four.
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